Rapid Response - Fentanyl Test Strip 2.0

Overview

The BTNX Fentanyl test strip is a colorimetric test for fentanyl that uses lateral flow immunoassay technology. Testing a sample for Fentanyl takes about 5 minutes.

Accuracy and Limit of Detection

The manufacture reports the test has an accuracy rate of 98% and a detection limit of this test is below 200 ng/mL (200 billionths of a gram).

Fentanyl Analogues

The manufacture instruction report the test will indicate positive for ~~many~~some Fentanyl analogues such as Carfentanil, Acetyl Fentanyl, ~~Butyryl Fentanyl, Remifentanil, Ocfentanil, Sufentanil, p-Fluoro Fentanyl, Furanyl Fentanyl, Valeryl Fentanyl,~~ and ~~3-Methyl~~Butyryl Fentanyl.

Cross Sensitivity

The manufacture reports no cross reactivity to other opiates such as Morphine and Heroin.

Field experience with version 1.0 has shown the tests may indicate a false positive for Fentanyl when testing high purity methamphetamine and MDMA. The tests may indicate a false negative when testing high purity fentanyl. ~~The module Operations Considerations provides guidance on how to rule out false positive and false negatives.~~

Storage Temperature

The kit must be stored in a temperature of 2-30°C/36-86°FF.

Kit Contents

The test kit contains 5 fentanyl test strips, swabs, test tubes, extraction liquid, a test tube holder, instructions, and product insert.

Conducting a Test

Bring tests, samples, buer and/or controls to room temperature 59-86°F (15-30°C) before use.

Step 1 Option 1: Preparing Powder or Pill Samples

A. Add 5mL of water (1 teaspoon or about 1 bottle cap) of clean water to a clean container like a cooker, bottle cap, plastic bag or similar.

B. Mix the drugs thoroughly before testing. If testing a pill crush it completely. Powders or crushed pills should be mixed thoroughly in a plastic bag by pinching and shaking the bag for at least 30 seconds.

C.Mix the drugs with water. Open the pouch and remove the microscoop. Using the microscoop, collect one scoop (5-10mg) of the powdered drug and add it to the water. Stir with the microscoop untilthe drug is completely dissolved in the water.

Step 1 Option 2: Preparing Liquid Drug Residue Samples:

A.After the drug shot is prepared and taken into the syringe, the leftover residue can be tested for the presence of Fentanyl. Add 1mL of clean water to the container or cooker having the residue.

Step 2: Testing

Remove the test strip from the pouch. It's best to use the strip immediately after opening. Do not use the strip if the pouch has been opened for more than one hour. Do not throw away the pouch until
you have read the results.

B. Hold the test strip by the blue end where "FYL" is printed. Avoid touching the strip's white membrane. Dip the strip into the solution, submerging it up to the wavy lines but not past the solid line. Hold it there for 10-15 seconds.

C.Remove the strip from the solution and place it on a non-absorbent flat surface. Start the timer and wait for the colored line(s) to appear.

D.A negative result can be interpreted as soon as both the test (T), and control (C) lines appear. A positive result can be interpreted once the control line has appeared and the background has cleared to white, typically by 60 seconds. Do not read results after 10 minutes. Compare the results to the “Results Interpretation” section below or to the back of the pouch.